A Research Assistant II is needed in the Department of Pathology, Microbiology, and Immunology. The laboratory's primary focus is studying host-microbiota interactions during intestinal inflammation.
This position requires a highly motivated individual with a track record of success.
excellent oral and written communication skills
high-quality recordkeeping including ongoing maintenance of a well-structured laboratory notebook and laboratory inventory
excellent organization skills
drive, focus, and ability to set an efficient and productive agenda after guidance
Key Functions and Expected Performances:
This position is 70% laboratory maintenance and 30% laboratory research. General responsibilities include: handling mice in both regular and germ-free housing, dissection of the spleen, liver, and intestines from mice post mortem, prepare protein, RNA, and DNA from tissues, perform genotyping PCR analysis from DNA isolated from rodent tissues and feces, quantitate and perform quality control of protein, RNA and DNA isolated from rodent tissue, perform western blot analysis of protein samples and prepare RNA and DNA libraries for high throughput sequencing and qPCR, subclone DNA fragments into vectors, transform bacteria, plate bacteria for quantitation, and work with bacterial samples in an anaerobic chamber. Laboratory maintenance duties include: organization and maintenance of a substantial portion of the laboratory's breeding colony of genetically manipulated and germ-free mice, inventory supplies, anticipate supply needs, and re-order when necessary, maintain records of daily activities and experimental results, prepare common-use chemical solutions for experiments, change gas cylinders for anaerobe chamber and assist with general lab cleaning duties including autoclaving, filling water containers and cleaning dishes.
Bachelor's degree in Biological Sciences, Microbiology, Molecular Biology, Immunology, or a related field.
Minimal of 2year experience working in a laboratory setting.
Prior laboratory experience
Prior experience in mouse handling is highly desired
Prior experience with PCR (realtime and conventional) and other basic molecular biology technology and sterile cell culture
Prior experience in basic microbiology techniques such as plating bacteria is highly desired
Vanderbilt University Medical Center is home to Vanderbilt University Hospital, The Monroe Carell Jr. Children’s Hospital at Vanderbilt, the Vanderbilt Psychiatric Hospital and the Vanderbilt Stallworth Rehabilitation Hospital. These hospitals experienced more than 61,000 inpatient admissions during fiscal year 2015. Vanderbilt’s adult and pediatric clinics treated nearly 2 million patients during this same period. Vanderbilt University Hospital and the Monroe Carell Jr. Children’s Hospital at Vanderbilt are recognized again this year by U.S. News & World Report’s Best Hospitals as among the nation’s best with 18 nationally ranked specialties. Vanderbilt University Medical Center is world renowned because of the innovation, work ethic and collegiality of its employees. From our health care advances to our compassionate care, Vanderbilt owes its accomplishments and reputation to staff and faculty who bring skill and drive and innovation to the medical center day after day. World-leading academic departments and comprehensive centers of excellence pursue scientific discoveries and transformational educational and clinical advances across the entire spectrum of health and disease.As t...he largest employer in middle Tennessee, we welcome those who are interested in ongoing development in a caring, culturally sensitive and professional atmosphere. Most of us spend so much of our lives at work, we want to be part of maintaining a workplace in which people support one another and encourage reaching for excellence. Many high-achieving employees stay at Vanderbilt because of the professional growth they experience and because of their appreciation of Vanderbilt’s benefits, public events and discussions, athletic opportunities, beautiful setting and, above all, sense of community and purpose.Vanderbilt and its employees share a set of mutual expectations that have been created with productivity, legality, fairness and safety always in mind. We believe that our investment in training and compensating employees multiplies in value when we enable individuals to deliver their best performance for the benefit of us all.